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Lee,Sarah Hwa In; Barancelli,Giovana Verginia; Corassin,Carlos Humberto; Rosim,Roice Eliana; Coppa,Carolina Fernanda Sengling Cebin; Oliveira,Carlos Augusto Fernandes de. |
ABSTRACT This study aimed to investigate the effect of peracetic acid (PAA, 0.5%) on adherent cells of three strains of Listeria monocytogenes strains belonging to serotypes 4b and 1/2b that had been previously isolated from the environment of a Brazilian cheese plant. The assays were conducted using polystyrene microplates and stainless steel coupons and the adhered cells were treated with PAA for 60, 120 and 180 s. On stainless steel, biofilms were partially inactivated by PAA after 60 s and almost 100% of the cells were damaged within 180 s using epifluorescence microscopy with LIVE/DEAD® staining. On polystyrene microplates, PAA decreased (P<0.05) biofilm biomass produced by the three L. monocytogenes isolates at 60 s, when compared with controls... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peracetic acid (PAA)/ biofilm/effects; L. monocytogenes; Dairy plants/Brazil. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300610 |
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Cornu, M; Beaufort, A; Rudelle, S; Laloux, L; Bergis, H; Miconnet, N; Serot, Thierry; Delignette Muller, M. |
Salting and smoking are ancient processes for fish preservation. The effects of salt and phenolic smoke compounds on the growth rate of L. monocytogenes in cold-smoked salmon were investigated through physico-chemical analyses, challenge tests on surface of cold-smoked salmon at 4 degrees C and 8 degrees C, and a survey of the literature. Estimated growth rates were compared to predictions of existing secondary models, taking into account the effects of temperature, water phase salt content, phenolic content, and additional factors (e.g. pH, lactate, dissolved CO2). The secondary model proposed by Devlieghere et al. [Devlieghere, F., Geeraerd, A.H., Versyck, K.J., Vandewaetere, B., van Impe, J., Debevere, J., 2001. Growth of Listeria monocytogenes in... |
Tipo: Text |
Palavras-chave: Phenolic compounds; Validation criteria; Microbial exposure assessment; Predictive microbiology; L. monocytogenes; Fishery products. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-3953.pdf |
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Khan,J.A.; Rathore,R.S.; Khan,S.; Ahmad,I.. |
Among current in vitro methods for identification of pathogenic Listeria monocytogenes (L. monocytogenes) rely on growth in culture media, followed by isolation, and biochemical and serological identification. Now PCR (Polymerase Chain Reaction) has been used for the rapid, sensitive and specific detection of pathogenic L. monocytogenes. The pathogenicity of the organism is highly correlated with haemolytic factor known as listeriolysin O (LLO). A total of 400 samples from meat and 250 samples from raw milk and their products were collected from various local dairy farms, dairy units and butcheries in Bareilly, India. Pure isolates of L. monocytogenes obtained after enrichment in Buffered Listeria enrichment broth (BLEB) followed by plating onto Listeria... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L. monocytogenes; Haemolysis; PCR; Vero cytotoxicity. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300013 |
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Monteiro,Lilyan Rosmery Luizaga de; Mesquita,Albenones José de; André,Maria Cláudia Dantas Porfirio Borges; Cardoso,Juliana Lamaro. |
Listeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-field gel electrophoresis (PFGE) types isolated from meat products collected at public markets in Araguaina city, TO. Sixty samples of raw ground beef and frescal sausage were analyzed during the second half of 2008. Five out of 30 samples (16.7%) of raw ground beef tested positive for L. monocytogenes, three of which were classified as serotype 1/2b and two as serotype 4b. Among the 30 samples of sausage collected, two strains of L. monocytogenes were isolated (6.7%), one of them belonging to serotype 1/2a and the other belonging to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulsed field gel electrophoresis; Ground beef; Frescal sausage; L. monocytogenes. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000800016 |
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